CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1) antibody

Antigen: CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1)

Synonyms: ctdsp1, MGC79551, CTDSP1, fj83f12, MGC113169, im:7141428, wu:fj83f12, zgc:113169

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190811

Additional Information

Alternative name: CTDSP1

Gene ID: 58190, 227292 (mouse), 363249 (rat)

Immunogen: C-RGKGDQKSAASQK

Format: Affinity-purified

Description: All names and symbols: CTDSP1, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 , NLIIF, SCP1 , nuclear LIM interactor-interacting factor, small CTD phosphatase 1. Official Symbol: CTDSP1. RefSeq number(s): NP_067021.1, NP_872580.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_067021.1and NP_872580.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 60kDa band in human spleen, lung and placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 29.2kDa according to NP_067021.1 and 29.1kDa according to NP_872580.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Zhang, Kim, Genoud et al.: "Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1." in: Molecular cell, Vol. 24, Issue 5, pp. 759-70, 2006 (PubMed).