CTDSP1 antibody (C-Term)

Catalog No. ABIN392888

Product Details anti-CTDSP1 Antibody

Target: CTDSP1 (CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1))

Binding Specificity: AA 235-261, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTDSP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CTDSP1-V250 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the C-terminal region of human CTDSP1-V250.

Clone: RB5996

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for CTDSP1

Target: CTDSP1 (CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1))

Alternative Name: CTDSP1 (CTDSP1 Products)

Synonyms: CTDSP1 antibody, NIF3 antibody, NLI-IF antibody, NLIIF antibody, SCP1 antibody, fj83f12 antibody, im:7141428 antibody, wu:fj83f12 antibody, zgc:113169 antibody, GIP antibody, Nif3 antibody, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 antibody, CTD small phosphatase 1 antibody, carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 antibody, CTDSP1 antibody, Ctdsp1 antibody, ctdsp1 antibody, HCAG_04820 antibody, CTRG_06220 antibody

Background: CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF, 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.

Molecular Weight: 29203

Gene ID: 58190

NCBI Accession: NP_001193807, NP_067021, NP_872580

UniProt: Q9GZU7