O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody

Antigen: O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT)

Synonyms: HRNT1, FLJ23071, MGC22921, O-GLCNAC, MGC80426, MGC69550

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190863

Additional Information

Alternative name: OGT

Gene ID: 8473, 108155 (mouse), 26295 (rat)

Immunogen: C-YEHPKDLKLSDGR

Format: Affinity-purified

Description: All names and symbols: OGT, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), FLJ23071, HRNT1, MGC22921, O-GLCNAC , O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase. Official Symbol: OGT. RefSeq number(s): NP_858058.1, NP_858059.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1 Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 110kDa band observed in Pancreas lysates from Rat and Mouse (calculated MW of 117kDa according to NP_858058.1. Recommended concentration: 0.05-0.2µg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190863 (0.05µg/ml) staining of Rat Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Majumdar, Wright, Markowitz et al.: "Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1." in: Diabetes, Vol. 53, Issue 12, pp. 3184-92, 2004 (PubMed).