OGT antibody (N-Term)

Catalog No. ABIN6972463

Product Details anti-OGT Antibody

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OGT antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Characteristics: OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression. OGT/O-GlcNAc transferase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a recombinant protein corresponding to the N-terminal region of human OGT.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: OGT/O-GlcNAc transferase (OGT Products)

Synonyms: BcDNA:GH04245 antibody, CG10392 antibody, Dmel\\CG10392 antibody, OGT antibody, Ogt antibody, P1201 antibody, SXC antibody, Sxc antibody, l(2)02637 antibody, l(2)NC130 antibody, sxc/Ogt antibody, fm81g08 antibody, ogt antibody, wu:fc12b01 antibody, wu:fm81g08 antibody, HRNT1 antibody, O-GLCNAC antibody, 1110038P24Rik antibody, 4831420N21Rik antibody, AI115525 antibody, Ogtl antibody, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog antibody, super sex combs antibody, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 antibody, O-linked N-acetylglucosamine (GlcNAc) transferase antibody, O-linked GlcNAc transferase antibody, o-linked GlcNAc transferase antibody, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody, ogt.L antibody, sxc antibody, ogt.1 antibody, OGT antibody, ogt antibody, GL50803_12081 antibody, GL50803_41701 antibody, THAPSDRAFT_264441 antibody, spyA antibody, Ogt antibody

Molecular Weight: 120 kDa

NCBI Accession: NP_858058

Pathways: Regulation of Carbohydrate Metabolic Process