This antibody detects Murine type VII Collagen (aa 757-967). Cross reacts with Human type VII Collagen. Not tested for reactivity with type VII Collagen from other species.
Purification
Protein G Chromatography
Immunogen
Recombinant Murine type VII Collagen (mixture of fragments aa 97-200, aa 479-578, aa 757-967) GST fusion protein. Genename: COL7A1
COL7
Reactivity: Mouse
IF (cc), IF (p)
Host: Rabbit
Polyclonal
Cy3
Application Notes
ELISA (1/5.000). Western blot (1/500). Immunocytochemistry on Frozen Sections (1/100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in aqua bidest to a concentration of 1 mg/mL.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store lyophilized at 2-8 °C and reconstituted at -20 °C.
COL7A1 antibody, collagen type VII alpha 1 chain antibody, collagen, type VII, alpha 1 antibody, COL7A1 antibody, col7a1 antibody
Background
Type VII Collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.Synonyms: COL7A1, LC collagen, Long-chain collagen