HLA Class I antibody (PE)

Antigen: HLA Class I

Clonality: Monoclonal (MEM-147)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN192165

Additional Information

Alternative name: HLA-Class I

Immunogen: PHA-activated peripheral blood lymphocytes

Isotype: IgG1

Clone: MEM-147

Description: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta2microglobulin. They carry foreign determinants important for immune recognition bycytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class Iantigens are represented by HLA-A, HLA-B and HLA-C molecules.

Specificity: The antibody MEM-147 reacts with all human classical MHC Class I molecules in nativecell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry andimmunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia)are expressed on the surface of all human nucleated cell types.The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only withmost HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classicalantibody W6/32.

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 0.1 mg/ml

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Publications

Publications: Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).