Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B) antibody (FITC)

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B)

Synonyms: DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5, TNFRSF10B, dr5, cd262, killer, trick2, trickb, xDR-M1, xDR-M2, ztnfr9, trailr2, trick2a, trick2b, MGC88957

Clonality: Monoclonal (DR5-01-1)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN192358

Additional Information

Alternative name: CD262 / TRAIL-R2

Immunogen: Recombinant fusion protein of human IgG heavy chain and extracellular domain of DR5.

Isotype: IgG1

Clone: DR5-01-1

Description: TRAIL-R2 (CD262, DR5) is one of two TNF superfamily member intracellular death domaincontaining receptors for TRAIL (APO2L).Apoptosis, or programmed cell death, occurs during normal cellular differentiation anddevelopment of multicellular organisms. Apoptosis is induced by certain cytokines includingtumor necrosis factor (TNF) and Fas ligand in the TNF family through their death domaincontaining receptors, TNF receptor 1 (TNFR1) and Fas, respectively. Another member inthe TNF family has been identified and designated TRAIL (for TNF related apoptosisinducing ligand) and Apo2L (for Apo2 ligand). Receptors for TRAIL include two deathdomain containing receptors, DR4 and DR5, as well as two decoy receptors, DcR1 andDcR2, lacking the intracellular signaling death domain. DcR1 (also called TRID), like therelated death receptors DR4 and DR5, contains two extracellular cysteine rich domains.However, DcR1 contains no intracellular death domain and is thus incapable of signalingapoptosis. It has been suggested DcR1 is responsible for TRAIL resistance in normalhuman tissues including heart, placenta, lung, liver, kidney, spleen, and bone marrow. DR5is a member of the TNF receptor superfamily, and contains an intracellular death domain.This receptor can be activated by tumor necrosis factor related apoptosis inducing ligand(TNFSF10/TRAIL/APO2L), and transduces apoptosis signal. Studies with FADD deficientmice suggested that FADD, a death domain containing adaptor protein, is required for theapoptosis mediated by this protein.

Specificity: The antibody DR5-01-1 recognizes an extracellular domain of TRAIL-R2 (DR5). TRAIL-R2is one of two TNF superfamily member intracellular death domain containing receptors forTRAIL (APO2L).

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 0.1 mg/ml

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only