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MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (pThr394)

Details for Product anti-MAP2K2 Antibody No. ABIN196623, Supplier: Login to see
Antigen
Alternatives
anti-Human MAP2K2 antibody for Western Blotting
Epitope
pThr394
37
24
15
15
14
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10
8
8
5
5
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3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
245
113
95
8
4
3
2
2
Host
Rabbit
198
45
9
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
10
10
9
7
7
7
3
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
202
84
81
44
33
27
24
17
15
12
11
4
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
Specificity MEK2 (phospho-Thr394) antibody detects endogenous levels of MEK2 only when phosphorylated at threonine 394.
Purification Immunoaffinity chromatography
Alternative Name MAPKK 2 (MAP2K2 Antibody Abstract)
Background Microtubule associated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2.Synonyms: Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, MEK2, MKK2
Gene ID 5605
NCBI Accession NP_109587
UniProt P36507
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02 % Sodium Azide as preservative and 50 % glycerol as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (pThr394) (ABIN196623) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (pThr394) antibody
 image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (pThr394) (ABIN196623) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (pThr394) antibody (Image 2)
 image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (pThr394) (ABIN196623) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (pThr394) antibody (Image 3)
Background publications Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).