JunD antibody (pSer255)

Catalog No. ABIN196660

Product Details anti-JunD Antibody

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Binding Specificity: pSer255

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. JunD (phospho-Ser255) antibody detects endogenous levels of JunD only when phosphorylated at serine 255.

Purification: Immunoaffinity chromatography

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human JunD around the phosphorylation site of serine 255 (G-E-SP-P-P).

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry on paraffinsections (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for JunD

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Alternative Name: Jun-D (JUND Products)

Synonyms: si:dkey-251j8.3 antibody, AP-1 antibody, Jund1 antibody, JunD proto-oncogene, AP-1 transcription factor subunit antibody, jun D proto-oncogene S homeolog antibody, jun D proto-oncogene antibody, jund antibody, jund.S antibody, JUND antibody, Jund antibody

Background: JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.Synonyms: JUND, Transcription factor jun-D

Gene ID: 3727

NCBI Accession: NP_005345

UniProt: P17535