Jun D Proto-Oncogene (JUND) (Ser255) antibody

Details for Product No. ABIN197142, Supplier: Log in to see
Antigen
  • si:dkey-251j8.3
  • AP-1
  • Jund1
  • JunD proto-oncogene, AP-1 transcription factor subunit
  • jun D proto-oncogene S homeolog
  • jun D proto-oncogene
  • jund
  • jund.S
  • JUND
  • Jund
Epitope
Ser255
53
21
15
15
6
5
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
142
81
68
11
8
7
6
6
4
2
1
1
1
1
1
Host
Rabbit
144
11
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
124
63
49
26
26
18
18
9
6
5
2
2
2
1
1
1
1
Options
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Immunogen Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of serine 255 (G-E-Sp-P-P).
Specificity JunD antibody detects endogenous levels of total JunD protein.
Purification Affinity-chromatography using epitope-specific immunogen
Alternative Name Jun-D (JUND Antibody Abstract)
Background JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.Synonyms: JUND, Transcription factor jun-D
Gene ID 3727
NCBI Accession NP_005345
UniProt P17535
Application Notes Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS( without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody (ABIN197142) anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody
 image for anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody (ABIN197142) anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody (Image 2)
 image for anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody (ABIN197142) anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody (Image 3)
 image for anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody (ABIN197142) anti-Jun D Proto-Oncogene (JUND) (Ser255) antibody (Image 4)
Background publications Beausoleil, Jedrychowski, Schwartz, Elias, Villén, Li, Cohn, Cantley, Gygi: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

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