Nibrin antibody (pSer343)

Catalog No. ABIN196715

This Rabbit Polyclonal antibody specifically detects Nibrin in WB. It exhibits reactivity toward Human.

Quick Overview for Nibrin antibody (pSer343) (ABIN196715)

Target: Nibrin (NBN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nibrin Antibody

Binding Specificity: pSer343

Specificity: The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. This antibody detects endogenous levels of p95/NBS1 only when phosphorylated at Serine 343.

Purification: Affinity Chromatography using epitope-specific peptide

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human p95/NBS1 around the phosphorylation site of Serine 343 (S-L-Sp-Q-G).

Application Details

Application Notes: Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: Nibrin

Background: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.Synonyms: Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95

Gene ID: 4683

NCBI Accession: NP_002476

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response