Rabbit anti-Mouse IgG Antibody (Biotin)

Catalog No. ABIN1967534

Product Details

Target: IgG

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: The antibody shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.

Purification: Immunoaffinity purified

Immunogen: Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:20000 - 1:100000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-Mouse IgG subclass pan reactive Secondary Antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody