Rabbit anti-Mouse IgG Antibody (Biotin)
-
- Target See all IgG products
- IgG
-
Reactivity
- Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Biotin
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- The antibody shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
- Purification
- Immunoaffinity purified
- Immunogen
-
Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.
Type of Immunogen: Purified protein
-
-
- Application Notes
-
Approved: ELISA (1:20000 - 1:100000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)
Usage: Anti-Mouse IgG subclass pan reactive Secondary Antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
-
Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA
- Handling Advice
- avoid freeze thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
-