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Paxillin antibody (PXN) (pTyr118)

Details for Product anti-PXN Antibody No. ABIN196776, Supplier: Login to see
Antigen
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • DPaxillin
  • DPxn
  • DPxn37
  • Dmel\\CG31794
  • Dpax
  • DpaxA
  • PDLP
  • dPax
  • pax
  • PXN
  • LOC100220858
  • AW108311
  • AW123232
  • Pax
  • wu:fw71f12
Alternatives
anti-Human Paxillin antibody for Western Blotting
Epitope
pTyr118
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53
28
24
23
18
14
13
9
9
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2
2
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2
1
1
1
1
1
1
1
1
1
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1
1
1
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1
Reactivity
Human, Mouse (Murine)
385
165
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27
6
3
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2
1
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Host
Rabbit
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2
Clonality
Polyclonal
Conjugate
This Paxillin antibody is un-conjugated
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5
2
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1
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
311
106
92
58
54
54
31
30
8
8
4
2
2
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Paxillin antibody (Phospho-Tyr118) detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.
Purification Immunoaffinity chromatography.
Alternative Name Paxillin / PXN (PXN Antibody Abstract)
Background Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).Synonyms: FLJ16691
Gene ID 5829
NCBI Accession NP_001074324
UniProt P49023
Pathways MAPK Signaling, EGFR Signaling Pathway
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-Paxillin antibody (PXN) (pTyr118) (ABIN196776) anti-Paxillin (PXN) (pTyr118) antibody
 image for anti-Paxillin antibody (PXN) (pTyr118) (ABIN196776) anti-Paxillin (PXN) (pTyr118) antibody (Image 2)
Background publications Tao, Wollscheid, OBrien et al.: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005 (PubMed).

Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Sanders, Basson: "p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23516-22, 2005 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).