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Paxillin (PXN) (pTyr118) antibody

Details for Product No. ABIN196776
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Antigen
Synonyms CG18061, CG18576, CG31794, CT40481, CT42454, DPaxillin, DPxn, DPxn37, Dmel\\CG31794, Dpax, DpaxA, PDLP, dPax, pax, PXN, LOC100220858, AW108311, AW123232, Pax, wu:fw71f12
Epitope
pTyr118
(45), (40), (29), (25), (21), (18), (14), (10), (9), (7), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(308), (171), (162), (48), (39), (25), (2), (1), (1)
Host
Rabbit
(304), (32), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(245), (90), (70), (63), (61), (40), (24), (17), (4), (4), (3), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN196776
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Paxillin antibody (Phospho-Tyr118) detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.
Purification Immunoaffinity chromatography.
Alternative Name Paxillin / PXN
Background Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).
Alternate names: FLJ16691
Gene ID 5829
NCBI Accession NP_001074324.1
UniProt P49023
Application Notes Suitable for use in Western blot (1: 500~1: 1000) and Immunohistochemistry (1: 50~1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-Paxillin (PXN) (pTyr118) antibody anti-Paxillin (PXN) (pTyr118) antibody
anti-Paxillin (PXN) (pTyr118) antibody (2) anti-Paxillin (PXN) (pTyr118) antibody (Image 2)
Background publications Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Sanders, Basson: "p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23516-22, 2005 (PubMed).

Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Tao, Wollscheid, OBrien et al.: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005 (PubMed).

Validation Images
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