Paxillin antibody (pTyr118)

Catalog No. ABIN196777

Product Details anti-Paxillin Antibody

Target: Paxillin (PXN)

Binding Specificity: pTyr118

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Paxillin antibody (Phospho-Tyr118) detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin / PXN (PXN Products)

Synonyms: AW108311 antibody, AW123232 antibody, Pax antibody, CG18061 antibody, CG18576 antibody, CG31794 antibody, CT40481 antibody, CT42454 antibody, DPaxillin antibody, DPxn antibody, DPxn37 antibody, Dmel\\CG31794 antibody, Dpax antibody, DpaxA antibody, PDLP antibody, dPax antibody, pax antibody, wu:fw71f12 antibody, PXN antibody, LOC100220858 antibody, paxillin antibody, Paxillin antibody, paxillin S homeolog antibody, paxillin a antibody, PXN antibody, Pxn antibody, Pax antibody, pxn.S antibody, pxna antibody, LOC696336 antibody, pxn antibody

Background: Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).Synonyms: FLJ16691

Gene ID: 5829

NCBI Accession: NP_001074324

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling