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Caveolin-1 antibody (Caveolin 1, Caveolae Protein, 22kDa) (pTyr14)

Details for Product anti-CAV1 Antibody No. ABIN196779, Supplier: Log in to see
Antigen
Epitope
pTyr14
37
28
20
11
9
8
7
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
230
110
104
24
17
9
9
8
8
6
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
184
35
32
3
Clonality
Polyclonal
Conjugate
This Caveolin-1 antibody is un-conjugated
6
4
3
2
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
186
65
53
50
48
29
19
9
5
5
4
2
2
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Caveolin-1 around the phosphorylation site of tyrosine 14 (H-L-YP-T-V).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Antibody AP02388PU detects endogenous levels of Caveolin-1 only when phosphorylated at Tyrosine 14.
Purification Immunoaffinity Chromatography.
Alternative Name Caveolin-1 (CAV1 Antibody Abstract)
Background Caveolae (also known as plasmalemmal vesicles) are 50-100 nm flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.Synonyms: CAV1
Gene ID 857
NCBI Accession NP_001744
UniProt Q03135
Research Area Signaling
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-Caveolin-1 antibody (Caveolin 1, Caveolae Protein, 22kDa) (pTyr14) (ABIN196779) anti-Caveolin 1, Caveolae Protein, 22kDa (CAV1) (pTyr14) antibody
 image for anti-Caveolin-1 antibody (Caveolin 1, Caveolae Protein, 22kDa) (pTyr14) (ABIN196779) anti-Caveolin 1, Caveolae Protein, 22kDa (CAV1) (pTyr14) antibody (Image 2)
Background publications Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Labrecque, Nyalendo, Langlois et al.: "Src-mediated tyrosine phosphorylation of caveolin-1 induces its association with membrane type 1 matrix metalloproteinase." in: The Journal of biological chemistry, Vol. 279, Issue 50, pp. 52132-40, 2004 (PubMed).

Fielding, Chau, Liu et al.: "Mechanism of platelet-derived growth factor-dependent caveolin-1 phosphorylation: relationship to sterol binding and the role of serine-80." in: Biochemistry, Vol. 43, Issue 9, pp. 2578-86, 2004 (PubMed).

Labrecque, Royal, Surprenant et al.: "Regulation of vascular endothelial growth factor receptor-2 activity by caveolin-1 and plasma membrane cholesterol." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 334-47, 2003 (PubMed).

Maggi, Biedi, Segat et al.: "IGF-I induces caveolin 1 tyrosine phosphorylation and translocation in the lipid rafts." in: Biochemical and biophysical research communications, Vol. 295, Issue 5, pp. 1085-9, 2002 (PubMed).