Rabbit anti-Mouse IgG Antibody (PE)
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- Target See all IgG products
- IgG
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- PE
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- The antibody shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
- Purification
- Immunoaffinity purified
- Immunogen
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Anti-Mouse IgG subclass pan reactive Secondary Antibody was produced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.
Type of Immunogen: Purified protein
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Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB, FACS, FLISA, FM, IP, LF Polyclonal IgG unconjugated
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
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- Application Notes
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Approved: Flo, IF (1:100 - 1:250), IHC
Usage: Anti-Mouse IgG subclass pan reactive Secondary Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA
- Handling Advice
- avoid freeze thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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