PKC mu antibody (pSer910)

Catalog No. ABIN196784

The Rabbit Polyclonal anti-PKC mu antibody is suitable to detect PKC mu in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for PKC mu antibody (pSer910) (ABIN196784)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC mu antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PKC mu Antibody

Binding Specificity: pSer910

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. This antibody detects endogenous levels of PKD/PKCμ only when phosphorylated at Serine 910.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCmy around the phosphorylation site of serine 910 (R-V-SP-I-L).

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Alternative Name: PRKD1

Background: Synonyms: PKC D1, PKC mu, PKD, PKD1, PRKCM, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, nPKC-D1, nPKC-mu

Gene ID: 5587

NCBI Accession: NP_002733

UniProt: Q15139

Pathways: Myometrial Relaxation and Contraction