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PKC mu antibody (AA 233-246)

Verified PRKD1 Reactivity: Human WB, ELISA, IHC, IP Host: Goat Polyclonal unconjugated
Catalog No. ABIN5539575
  • Target See all PKC mu (PRKD1) Antibodies
    PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))
    Binding Specificity
    • 27
    • 15
    • 15
    • 15
    • 14
    • 8
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 233-246
    Reactivity
    • 133
    • 85
    • 73
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 147
    • 2
    • 1
    Goat
    Clonality
    • 140
    • 10
    Polyclonal
    Conjugate
    • 67
    • 10
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    This PKC mu antibody is un-conjugated
    Application
    • 101
    • 51
    • 40
    • 40
    • 33
    • 18
    • 15
    • 10
    • 6
    • 5
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Purpose
    PRKD1 (aa233-246)
    Sequence
    QKSPSESFIG REKR
    Cross-Reactivity
    Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
    Top Product
    Discover our top product PRKD1 Primary Antibody
  • Application Notes

    Immunohistochemistry: This antibody has been confirmed in human pancreatic adenocarcinoma (PDA) according to Döppler et al, Sci Rep. 2016 Sep 21,6:33758 PMID 27649783.

    Western Blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100 kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))
    Alternative Name
    PRKD1 (PRKD1 Products)
    Synonyms
    MGC83727 antibody, PRKD1 antibody, pkd antibody, pkcm antibody, prkcm antibody, pkc-mu antibody, PKC-MU antibody, PKCM antibody, PKD antibody, PRKCM antibody, PKD1 antibody, Pkcm antibody, Prkcm antibody, nPKC-D1 antibody, nPKC-mu antibody, PKC antibody, protein kinase D1 antibody, protein kinase D1 L homeolog antibody, PRKD1 antibody, prkd1.L antibody, prkd1 antibody, Prkd1 antibody
    Background
    PRKD1, PKC-MU, PKCM, PKD, PRKCM,
    Gene ID
    5587, 18760, 85421
    NCBI Accession
    NP_002733
    Pathways
    Myometrial Relaxation and Contraction
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