CDC25C antibody (pSer216)

Catalog No. ABIN196818

The Rabbit Polyclonal anti-CDC25C antibody (ABIN196818) specifically detects CDC25C in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CDC25C antibody (pSer216) (ABIN196818)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC25C Antibody

Binding Specificity: pSer216

Specificity: This antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Serine 216 (S-P-Sp-M-P).

Application Details

Application Notes: Suitable for use in Immunohistochemistry (1/50approx. 1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25C

Background: Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.Synonyms: Cell Division Cycle 25C, Dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3

Gene ID: 995

NCBI Accession: NP_001781

UniProt: P30307

Pathways: Cell Division Cycle, M Phase