LIM Domain Kinase 1 antibody (LIMK1) (pThr508)

Details for Product anti-LIMK1 Antibody No. ABIN196830, Supplier: Log in to see
Antigen
  • limk1
  • xlimk1
  • GB16654
  • LIMK1
  • LIMK
  • LIMK-1
  • LIM domain kinase 1
  • LIM domain kinase 1a
  • LIM-kinase1
  • LIM-domain containing, protein kinase
  • LIMK1
  • limk1a
  • limk1
  • CpipJ_CPIJ000717
  • Limk1
Alternatives
anti-Rat (Rattus) LIM Domain Kinase 1 antibody for ELISA
Epitope
pThr508
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2
1
1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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97
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5
5
3
2
2
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2
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1
1
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1
Host
Rabbit
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70
3
Clonality
Polyclonal
Conjugate
This LIM Domain Kinase 1 antibody is un-conjugated
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7
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1
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Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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17
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5
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1
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).
Specificity This antibody detects endogenous levels of LIMK1 only when phosphorylated at Threonine 508.
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK1 (LIMK1 Antibody Abstract)
Background LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. LIMK1 may be involved in brain development, it is highly expressed in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1
Gene ID 3984
NCBI Accession NP_001191355
UniProt P53667
Research Area Neurology, Tyrosine Kinases, Cytoskeleton, Microfilaments
Pathways Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-LIM Domain Kinase 1 antibody (LIMK1) (pThr508) (ABIN196830) anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
 image for anti-LIM Domain Kinase 1 antibody (LIMK1) (pThr508) (ABIN196830) anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody (Image 2)
 image for anti-LIM Domain Kinase 1 antibody (LIMK1) (pThr508) (ABIN196830) anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody (Image 3)
Background publications Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Edwards, Sanders, Bokoch, Gill: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).