MEK1 antibody (pSer218, pSer221)

Catalog No. ABIN196945

The Rabbit Polyclonal anti-MEK1 antibody (ABIN196945) specifically detects MEK1 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MEK1 antibody (pSer218, pSer221) (ABIN196945)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MEK1 Antibody

Binding Specificity: pSer218, pSer221

Specificity: This antibody detects endogenous levels of MEK1/MEK2 only when phosphorylated at Serine 217/221.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around the phosphorylation site of Serine 217/221 (I-D-Sp-M-A) derived from Human MEK1/MEK2

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl and 50 % Glycerol, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1

Background: Synonyms: Dual specificity mitogen-activated protein kinase kinase 1/2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 1, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK1, MEK1, MEK2, PRKMK1, PRKMK2

Molecular Weight: 45 kDa

Gene ID: 5604

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling