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NFKBIE antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon) (pSer22)

Details for Product anti-NFKBIE Antibody No. ABIN196949, Supplier: Log in to see
Antigen
Alternatives
anti-Human NFKBIE antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
pSer22
35
13
13
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
93
55
36
5
1
Host
Rabbit
94
2
Clonality
Polyclonal
Conjugate
This NFKBIE antibody is un-conjugated
4
4
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
60
36
33
18
12
9
3
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IkB- epsilon around the phosphorylation site of serine 22 ( I-E-SP-L-R).
Specificity IkB-epsilon antibody detects endogenous levels of IkB-epsilon only when phosphorylated at serine 22.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name NFKBIE / IKBE (NFKBIE Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.Synonyms: I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon, NF-kappa-B inhibitor epsilon
Gene ID 4794
NCBI Accession NP_004547
UniProt O00221
Pathways NF-kappaB Signaling
Application Notes Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-NFKBIE antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon) (pSer22) (ABIN196949) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE) (pSer22) antibody
 image for anti-NFKBIE antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon) (pSer22) (ABIN196949) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE) (pSer22) antibody (Image 2)
Background publications McDevitt: "Discovering the role of the major histocompatibility complex in the immune response." in: Annual review of immunology, Vol. 18, pp. 1-17, 2000 (PubMed).

Shirane, Hatakeyama, Hattori et al.: "Common pathway for the ubiquitination of IkappaBalpha, IkappaBbeta, and IkappaBepsilon mediated by the F-box protein FWD1." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28169-74, 1999 (PubMed).

Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996 (PubMed).

Brown, Gerstberger, Carlson et al.: "Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1485-8, 1995 (PubMed).

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