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Ezrin (EZR) (Tyr353) antibody

Details for Product No. ABIN197259, Supplier: Log in to see
Antigen
Epitope
Tyr353
28
28
23
23
22
21
14
14
11
8
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
317
178
156
13
10
8
5
4
3
3
3
3
2
2
Host
Rabbit
260
31
25
2
Clonality
Polyclonal
Conjugate
Un-conjugated
11
9
8
5
5
5
4
4
4
4
4
4
2
1
1
Application
Western Blotting (WB)
249
103
62
45
41
23
6
5
5
3
3
1
1
1
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
Specificity Ezrin antibody detects endogenous levels of total Ezrin protein.
Purification Affinity-chromatography using epitope-specific immunogen
Alternative Name Ezrin (EZR Antibody Abstract)
Background Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81
Gene ID 7430
NCBI Accession NP_001104547
UniProt P15311
Research Area Cancer, Cytoskeleton, Microfilaments
Pathways
Application Notes Western Blot: 1: 500approx. 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-Ezrin (EZR) (Tyr353) antibody (ABIN197259) anti-Ezrin (EZR) (Tyr353) antibody
Background publications Zhao, Shiue, Palkon et al.: "Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9485-90, 2004 (PubMed).

Wang, Pfeiffer, Gaarde: "Activation of SRC tyrosine kinases in response to ICAM-1 ligation in pulmonary microvascular endothelial cells." in: The Journal of biological chemistry, Vol. 278, Issue 48, pp. 47731-43, 2003 (PubMed).

Gautreau, Poullet, Louvard et al.: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, 1999 (PubMed).

Crepaldi, Gautreau, Comoglio et al.: "Ezrin is an effector of hepatocyte growth factor-mediated migration and morphogenesis in epithelial cells." in: The Journal of cell biology, Vol. 138, Issue 2, pp. 423-34, 1997 (PubMed).