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NFKBIE antibody (Ser22)

NFKBIE Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN197570
  • Target See all NFKBIE Antibodies
    NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))
    Binding Specificity
    • 25
    • 15
    • 15
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser22
    Reactivity
    • 69
    • 48
    • 22
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 83
    • 2
    Rabbit
    Clonality
    • 75
    • 10
    Polyclonal
    Conjugate
    • 29
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This NFKBIE antibody is un-conjugated
    Application
    • 71
    • 39
    • 39
    • 22
    • 15
    • 11
    • 9
    • 6
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    IkB-ε antibody detects endogenous levels of total IkB-ε protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    The antiserum was produced against synthesized non-phosphopeptide derived from human IkB-epsilon around the phosphorylation site of serine 22 ( I-E-SP-L-R).
    Top Product
    Discover our top product NFKBIE Primary Antibody
  • Application Notes
    Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
  • Target
    NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))
    Alternative Name
    NFKBIE / IKBE (NFKBIE Products)
    Synonyms
    NFKBIE antibody, IKBE antibody, Slc35b2 antibody, NFKB inhibitor epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon antibody, NFKBIE antibody, Nfkbie antibody
    Background
    NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.Synonyms: I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon, NF-kappa-B inhibitor epsilon
    Gene ID
    4794
    NCBI Accession
    NP_004547
    UniProt
    O00221
    Pathways
    NF-kappaB Signaling, Maintenance of Protein Location
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