IKBKG antibody

Catalog No. ABIN199307

The Mouse Monoclonal anti-IKBKG antibody is suitable to detect IKBKG in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for IKBKG antibody (ABIN199307)

Target: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKBKG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IKBKG Antibody

Specificity: Recognizes IKKg/IKK3, Mr 48kD. IKKg/IKK3 is also known as NEMO, FIP-3 and IKKAP-1. Species cross-reactivity: Human and mouse.

Purification: Protein G purified

Immunogen: His-tagged, full-length human IKKg/IKK3.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, WB

Usage: Suitable for use in ELISA and Western Blot. Western Blot Analysis: 0.5-2 μg/mL detects IKKg/IKK3 in RIPA lysates from Jurkat and 3T3 cells. Jurkat lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IKKg/IKK3 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.01 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IKBKG

Target: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Alternative Name: IKBKG / NEMO / IKK Gamma

Background: Name/Gene ID: IKBKG
Subfamily: IKAPPAB
Family: Protein Kinase Non Catalytic

Synonyms: IKBKG, AMCBX1, FIP3, Fip3p, IkappaB kinase gamma, IKKgamma, IKK-gamma, NEMO, NF-kappa-B essential modifier, I-kappa-B kinase gamma-subunit, I-kappa-B kinase subunit gamma, IkB kinase gamma subunit, IKKAP1, Incontinentia pigmenti, IP2, IPD2, NF-kappa-B essential modulator, ZC2HC9, FIP-3, IkB kinase subunit gamma, IKKG, IP1, NFkappaB essential modulator

Gene ID: 8517

UniProt: Q9Y6K9

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins