S1A8 (AA 1-93) antibody

Catalog No. ABIN1993751

Product Details

Target: S1A8

Binding Specificity: AA 1-93

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Protein S100-A8 protein (1-93AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:200-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin^TM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details

Target: S1A8

Background:

Background: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect, regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread.

Aliases: 60B8Ag antibody, AI323541 antibody, B8Ag antibody, BEE11 antibody, CAGA antibody, Calgranulin-A antibody, Calprotectin L1L subunit antibody, Calprotectin, included antibody, CFAG antibody, CGLA antibody, Chemotactic cytokine CP-10 antibody, CP-10 antibody, Cystic fibrosis antigen antibody, L1Ag antibody, Leukocyte L1 complex light chain antibody, MA387 antibody, MIF antibody, Migration inhibitory factor-related protein 8 antibody, MRP-8 antibody, Myeloid-related protein 8 antibody, Neutrophil cytosolic 7 kDa protein antibody, NIF antibody, p8 antibody, Pro-inflammatory S100 cytokine antibody, Protein S100-A8 antibody, S100 calcium binding protein A8 (calgranulin A) antibody, S100 calcium binding protein A8 antibody, S100 calcium-binding protein A8 antibody, S100A8 antibody, S100A8/S100A9 complex, included antibody, S10A8_HUMAN antibody, Urinary stone protein band A antibody

UniProt: P05109