BUB1 antibody

Catalog No. ABIN1994737

Product Details anti-BUB1 Antibody

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purification

Immunogen: Recombinant protein of Human BUB1

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2000, IHC:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Store at -20 °C or -80 °C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1 (BUB1 Products)

Synonyms: bub1a antibody, bub1l antibody, hbub1 antibody, xbub1 antibody, BUB1A antibody, BUB1L antibody, hBUB1 antibody, AL022991 antibody, Bub1a antibody, C80208 antibody, D2Xrf87 antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, mitotic checkpoint serine/threonine kinase antibody, BUB1 antibody, bub1 antibody, Bub1 antibody

Background:

Background: The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions. During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase. Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers. Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome. Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility. Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores, and is required for functional microtubule-kinetochore interactions.

Aliases: Bub1 antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, S. cerevisiae, homolog of antibody, BUB1_HUMAN antibody, BUB1A antibody, BUB1L antibody, Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody, Budding uninhibited by benzimidazoles 1 homolog antibody, Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody, hBUB1 antibody, Homolog of mitotic checkpoint gene BUB1 antibody, Mitotic checkpoint gene BUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody, Mitotic spindle checkpoint kinase antibody, Putative serine/threonine protein kinase antibody