c-FOS antibody

Catalog No. ABIN1994798

Product Details anti-c-FOS Antibody

Target: c-FOS (c-Fos) (c-Fos)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-FOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purification

Immunogen: Center-peptide of Human FOS

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2000, IHC:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Store at -20 °C or -80 °C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: FOS (c-Fos Products)

Synonyms: AP-1 antibody, C-FOS antibody, p55 antibody, c-fos antibody, D12Rfj1 antibody, cFos antibody, cFOS antibody, fos antibody, Fos proto-oncogene, AP-1 transcription factor subunit antibody, FBJ osteosarcoma oncogene antibody, proto-oncogene c-Fos-like antibody, FOS antibody, Fos antibody, LOC101078177 antibody

Background:

Background: The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) . While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), that lacks the carboxy-terminal 101 amino acids . The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity . Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization . Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells . Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours . FRA1 and FRA2 expression persists longer and appreciable levels can be detected in asynchronously growing cells . Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation, however, Delta FosB lacks the ability to transform cells .

Aliases: Activator protein 1 antibody, AP 1 antibody, C FOS antibody, Cellular oncogene c fos antibody, Cellular oncogene fos antibody, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody, FBJ murine osteosarcoma viral oncogene homolog antibody, FBJ murine osteosarcoma viral v fos oncogene homolog antibody, FBJ Osteosarcoma Virus antibody, FOS antibody, FOS protein antibody, FOS_HUMAN antibody, G0 G1 switch regulatory protein 7 antibody, G0/G1 switch regulatory protein 7 antibody, G0S7 antibody, Oncogene FOS antibody, p55 antibody, proto oncogene c Fos antibody, Proto oncogene protein c fos antibody, Proto-oncogene c-Fos antibody, v fos FBJ murine osteosarcoma viral oncogene homolog antibody

Pathways: S100 Proteins