Complement C3c antibody

Catalog No. ABIN206657

Product Details anti-Complement C3c Antibody

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement C3c antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Specificity: Recognizes 135kD complement component 3c (C3c), present in blood serum. C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha chain fragment 1 + alpha chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation. Binds both C3c and C3 and so is not suitable for all ELISA applications.

Purification: Protein A purified

Immunogen: Native human C3 from normal serum, highly purified by chromatography.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, WB

Usage: ELISA: LS-C44974 binds both C3c and C3 and so is not suitable for all ELISA applications. Western Blot: LS-C44974 binds both C3c and C3 and so is not suitable for all ELISA applications.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for Complement C3c

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Alternative Name: Complement C3/C3c (C3C Products)

UniProt: P01024