CD16 antibody

Catalog No. ABIN206894

Product Details anti-CD16 Antibody

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut)

Specificity: Reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). Recognizes a polymorphism of CD16a (NK-cells form), it reacts well with granulocyte form (CD16b) of all subjects

Purification: Protein A purified

Immunogen: Human granulocytes.

Type of Immunogen: Cells

Clone: MEM-154

Isotype: IgG1

Application Details

Application Notes: Approved: Block, Flo (5 - 10 μg/mL), Func, IP (5 - 10 μg/mL), Neut, WB (5 - 10 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 5-10 μg/mL. Positive control - PBL (peripheral blood lymphocytes). Application note - The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Western Blotting: Application note - Non-redu The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide free PBS, preserved by filter sterilization, approx,  pH 7.4.

Preservative: Azide free

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD16

Target: CD16

Alternative Name: CD16 (CD16 Products)

Synonyms: Fcgr3 antibody, Fcgr3a antibody, CD16 antibody, FCGR3 antibody, FCGR3@ antibody, CD16b antibody, FCG3 antibody, FCR-10 antibody, FCRIII antibody, FCRIIIb antibody, FcRIII antibody, Fc fragment of IgG, low affinity IIa, receptor antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody, Fc fragment of IgG receptor IIIb antibody, Fc fragment of IgG receptor IIIa antibody, Fc receptor, IgG, low affinity III antibody, Fc-gamma RIII antibody, Fcgr2a antibody, FCGR3A antibody, FCGR3B antibody, Fcgr3 antibody, FCGR3 antibody

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process