PAPPA antibody
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- Target See all PAPPA Antibodies
- PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PAPPA antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid-treated PAPP-A. The epitope is different from those of clones 6F172 and 6F174 and is located on the PAPP-A subunit of the heterotetramer. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. This product detects only unreduced PAPP-A in Western Blot. It gives the strongest signal in Immunohistochemistry compared to clone 6F172 and clone 6F174.
- Purification
- Protein A/G purified
- Immunogen
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Purified human PAPP-A.
Type of Immunogen: Purified protein - Clone
- 1_24
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PAPPA Primary Antibody
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- Application Notes
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Approved: ELISA (1:8000), IHC, WB
Usage: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. Western Blot: Detects only unreduced PAPP-A. Immunohistochemistry: Gives the strongest signal compared to clone 6F172 and clone 6F174. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 15 mM sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))
- Alternative Name
- PAPPA / PAPP-A (PAPPA Products)
- Synonyms
- 8430414N03Rik antibody, IGFBP-4ase antibody, PAG1 antibody, PAPP-A antibody, ASBABP2 antibody, DIPLA1 antibody, PAPA antibody, PAPPA1 antibody, Pappa antibody, pappalysin 1 antibody, pregnancy-associated plasma protein A antibody, PAPPA antibody, Pappa antibody, Pappa1 antibody
- Background
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Name/Gene ID: PAPPA
Subfamily: Metallopeptidase M43
Family: Protease
Synonyms: PAPPA, DIPLA1, IGF-dependent IGFBP-4 protease, PAPPA1, PAPA, PAPP-A, Pappalysin-1, ASBABP2, IGFBP-4ase - Gene ID
- 5069
- UniProt
- Q13219
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