AIF antibody (AA 593-606)

Catalog No. ABIN211058

The Rabbit Polyclonal anti-AIF antibody is suitable to detect AIF in samples from Human, Rat, Mouse, Pig, Cow, Dog, Horse, Rabbit, Monkey, Hamster and Bat. It has been validated for WB.

Quick Overview for AIF antibody (AA 593-606) (ABIN211058)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Rat, Mouse, Pig, Cow, Dog, Horse, Rabbit, Monkey, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AIF Antibody

Binding Specificity: AA 593-606

Specificity: Recognizes AIF, Mr 57kD. Another non-specific protein was also detected at Mr 120kD. Species cross-reactivity: Mouse. Predicted for human and rat based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Cat, Horse, Rabbit, Pig (100%) Opossum, Xenopus (92%) Turkey, Chicken, Stickleback, Pufferfish, Zebrafish (83%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 593-606 of human Apoptosis Inducing Factor, AIF (C-KDGEQHEDLNEV-AK), containing a N-terminal cysteine. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Cat, Horse, Rabbit, Pig (100%), Opossum, Xenopus (92%), Turkey, Chicken, Stickleback, Pufferfish, Zebrafish (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot Analysis: 1 μg/mL detects AIF from a mouse liver preparation. Mouse liver protein preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with AIF (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 200 μg of protein A purified rabbit IgG in 200 μLof 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol. Liquid at -20 deg C.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1 / AIF / PDCD8

Background: Name/Gene ID: AIFM1
Family: Apoptosis

Synonyms: AIFM1, AIF, COXPD6, PDCD8, Programmed cell death 8

Gene ID: 9131

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect