NOG antibody (AA 28-232)
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- Target See all NOG Antibodies
- NOG (Noggin (NOG))
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Binding Specificity
- AA 28-232
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NOG antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to Noggin (NOG)
- Sequence
- MGHHHHHHSG S-QHY LHIRPAPSDN LPLVDLIEHP DPIFDPKEKD LNETLLRSLL GGHYDPGFMA TSPPEDRPGG GGGAAGGAED LAELDQLLRQ RPSGAMPSEI KGLEFSEGLA QGKKQRLSKK LRRKLQMWLW SQTFCPVLYA WNDLGSRFWP RYVKVGSCFS KRSCSVPEGM VCKPSKSVHL TVLRWRCQRR GGQRCGWIPI QYPIISECKC SC
- Specificity
- The antibody is a mouse monoclonal antibody raised against NOG. It has been selected for its ability to recognize NOG in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Noggin (NOG) corresdonding to Gln28~Cys232 (Accession # Q13253)
- Clone
- A7
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product NOG Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- NOG (Noggin (NOG))
- Alternative Name
- Noggin (NOG Products)
- Synonyms
- SYM1 antibody, SYNS1 antibody, nog-A antibody, nog1 antibody, noggin-1 antibody, noggin antibody, noggin antibody, noggin L homeolog antibody, noggin protein antibody, NOG antibody, Nog antibody, nog.L antibody, noggin antibody
- Background
- SYM1, SYNS1, Synostoses(Multiple)Syndrome 1, Symphalangism 1(Proximal)
- Pathways
- Stem Cell Maintenance, Tube Formation
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