Elastin antibody (AA 392-645)
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- Target See all Elastin (ELN) Antibodies
- Elastin (ELN)
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Binding Specificity
- AA 392-645
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Elastin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to Elastin (ELN)
- Sequence
- MGHHHHHHSG SEF- GGIPTYGVG AGGFPGFGVG VGGIPGVAGV PGVGGVPGVG GVPGVGISPE AQAAAAAKAA KYGVGTPAAA AAKAAAKAAQ FGLVPGVGVA PGVGVAPGVG VAPGVGLAPG VGVAPGVGVA PGVGVAPGIG PGGVAAAAKS AAKVAAKAQL RAAAGLGAGI PGLGVGVGVP GLGVGAGVPG LGVGAGVPGF GAVPGALAAA KAAKYGAAVP GVLGGLGALG GVGIPGGVVG AGPAAAAAAA KAAAKAAQFG LVGAA
- Specificity
- The antibody is a mouse monoclonal antibody raised against ELN. It has been selected for its ability to recognize ELN in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Rat
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Elastin (ELN) corresdonding to Gly392~Ala645 with N-terminal His Tag
- Clone
- C3
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ELN Primary Antibody
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- Application Notes
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Western blotting: 0.5-3 μg/mL
Immunohistochemistry: 5-30 μg/mL
Immunocytochemistry: 5-30 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Elastin (ELN)
- Alternative Name
- Elastin (ELN Products)
- Synonyms
- SVAS antibody, WBS antibody, WS antibody, AI385707 antibody, AI480567 antibody, E030024M20Rik antibody, RATTREL11 antibody, TREL11 antibody, Trela antibody, Trela26 antibody, ELN antibody, elastin antibody, ELN antibody, Eln antibody, LOC574132 antibody, Tcur_2481 antibody, Kfla_2289 antibody, LOC100620140 antibody
- Background
- WBS, WS, SVAS, Tropoelastin, Supravalvular Aortic Stenosis, Williams-Beuren Syndrome
- Pathways
- Regulation of Actin Filament Polymerization
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