Recombinant Elastin antibody

Catalog No. ABIN7477467

Product Details anti-Elastin Antibody

Target: Elastin (ELN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant full-length human Elastin protein

Clone: MSVA-648R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial).

Negative Control: Colon: Elastin immunostaining should be absent in all epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: Elastin (ELN Products)

Synonyms: SVAS antibody, WBS antibody, WS antibody, AI385707 antibody, AI480567 antibody, E030024M20Rik antibody, RATTREL11 antibody, TREL11 antibody, Trela antibody, Trela26 antibody, ELN antibody, elastin antibody, ELN antibody, Eln antibody, LOC574132 antibody, Tcur_2481 antibody, Kfla_2289 antibody, LOC100620140 antibody

Background: Elastin, ELN, SVAS, Tropoelastin, WBS, WS,Elastin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization