TBK1 antibody (AA 563-577)

Catalog No. ABIN213888

The Mouse Monoclonal anti-TBK1 antibody (Clone 108A429 ) (ABIN213888) specifically detects TBK1 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Pig, Monkey, Dog, Horse and Hamster samples.

Quick Overview for TBK1 antibody (AA 563-577) (ABIN213888)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig, Monkey, Dog, Horse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 108A429 

Product Details anti-TBK1 Antibody

Binding Specificity: AA 563-577

Specificity: A synthetic peptide corresponding to amino acids 563-577 YYQFKKDKAERRLAYC of human TBK1. GenBank no. NP-037386.1 was used as immunogen. This sequence is 100 % conserved between human, mouse, rat, dog and cow.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%) Elephant, Chicken, Platypus (93%) Bat, Turkey, Lizard (87%) Xenopus, Pufferfish, Zebrafish, Stickleback (80%).

Purification: Protein G purified

Immunogen: A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody. This sequence is 100% conserved between human, mouse, rat, dog and cow. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%), Elephant, Chicken, Platypus (93%), Bat, Turkey, Lizard (87%), Xenopus, Pufferfish, Zebrafish, Stickleback (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Alternative Name: NAK / TBK1

Background: Name/Gene ID: TBK1
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: TBK1, T2K, NAK, NF-kappa-B-activating kinase, NF-kB-activating kinase, TANK-binding kinase 1

Gene ID: 29110

UniProt: Q9UHD2

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome