MCL-1 antibody

Catalog No. ABIN214165

This Mouse Monoclonal antibody specifically detects MCL-1 in WB, ELISA, IHC, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MCL-1 antibody (ABIN214165)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 8C6D4B1

Product Details anti-MCL-1 Antibody

Specificity: Ni-NTA purified recombinant protein MCL-1expressed in E. Coli strain M15

Purification: Protein G purified

Immunogen: Purified recombinant fragment of human MCL-1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-Fr (1:200 - 1:1000), IHC-P (1:200 - 1:1000), WB (1:100 - 1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200-1:1000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Purified antibody in PBS containing 0.03 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: -20°C.

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1 / MCL 1

Background: Name/Gene ID: MCL1

Synonyms: MCL1, BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, Bcl2-L-3, MCL1S, MCL1L, Myeloid cell leukemia ES, MCL 1, MCL1-ES, TM, EAT, Mcl-1

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling