Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (FITC)

Catalog No. ABIN2145575

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Conjugate: FITC

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Characteristics: High purity Rabbit IgG(H+L) is prepared from serum of healthy rabbit. Affinity purified Goat anti- Rabbit IgG(H+L) isolated from pooled antiserum from goats hyperimmunized with purified Rabbit IgG(H+L).

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
• Add 2 mL1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add appropriate amount of fluorescent-labeled Goat anti Mouse IgG and incubate for 20 minutes away from light at room temperature.
• Repeat step 5.
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: aqueous buffer

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody