P2RX2 antibody (AA 55-104)

Catalog No. ABIN6736419

The Rabbit Polyclonal anti-P2RX2 antibody is suitable to detect P2RX2 in samples from Human, Mouse, Rat, Guinea Pig and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for P2RX2 antibody (AA 55-104) (ABIN6736419)

Target: P2RX2 (Purinergic Receptor P2X, Ligand Gated Ion Channel 2 (P2RX2))

Reactivity: Human, Mouse, Rat, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-P2RX2 Antibody

Binding Specificity: AA 55-104

Specificity: Human P2RX2 / P2X2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Guinea pig (100%) Dog, Bovine, Rabbit, Horse, Pig, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa55-104 of human P2RX2 (Q9UBL9, NP_733782). Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Guinea pig (100%), Galago, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Chicken, Platypus (92%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for P2RX2

Target: P2RX2 (Purinergic Receptor P2X, Ligand Gated Ion Channel 2 (P2RX2))

Alternative Name: P2RX2 / P2X2

Background: Name/Gene ID: P2RX2
Subfamily: P2X receptor
Family: Ion Channel

Synonyms: P2RX2, ATP receptor, p2X2, p2X Receptor, subunit 2, Purinergic receptor, p2RX2, p2X receptor 2, p2X purinoceptor 2, p2X2 purinoceptor

Gene ID: 22953

NCBI Accession: NP_733782

UniProt: Q9UBL9

Pathways: Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity