Desmoglein 2 antibody

Catalog No. ABIN215765

Product Details anti-Desmoglein 2 Antibody

Target: Desmoglein 2 (DSG2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Antigen Recognized in Species Human. Reactivity on Cultured Cell Lines Many, including CaCo-2, HaCaT, MCF-7. Recognizes desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide reacting: desmoglein 2 (1069aa, calculated mol. wt. 116760).

Purification: Protein A purified

Immunogen: Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain).

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr (1:10), IHC-P, WB

Usage: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Immunohistochemistry (Cryostat): 1:10 with PBS,  pH 7.4, Incubation: 1 hr at RT. Note: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2 % Triton X-100 for 5-10 min, depending on tissue type, is recommended. Immunohistochemistry (Paraffin): After microwave treatment, dilute 1:10 with PBS,  pH 7.4, Incubation: Overnight at 4?C.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: sterile ddH20

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 0.09 % sodium azide, 0.5 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Lyophilized and resuspended forms: store at -20°C short term.

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: DSG2 / Desmoglein 2 (DSG2 Products)

Synonyms: DSG2 antibody, dsc antibody, dsga antibody, si:dkeyp-51f12.6 antibody, wu:fj23f02 antibody, ARVC10 antibody, ARVD10 antibody, CDHF5 antibody, CMD1BB antibody, HDGC antibody, AA408168 antibody, D18Ertd293e antibody, desmoglein 2 antibody, desmoglein 2, tandem duplicate 1 antibody, DSG2 antibody, dsg2.1 antibody, dsg2 antibody, Dsg2 antibody

Background: Name/Gene ID: DSG2
Family: Cadherin

Synonyms: DSG2, ARVC10, Cadherin family member 5, CDHF5, CMD1BB, Desmoglein-2, Desmoglein II, HDGC, ARVD10, Desmoglein 2

Gene ID: 1829

UniProt: Q14126

Pathways: Cell-Cell Junction Organization