SLC6A3 antibody (N-Term)

Catalog No. ABIN215796

The Rabbit Polyclonal anti-SLC6A3 antibody (ABIN215796) specifically detects SLC6A3 in WB, ELISA, IHC, IP and IHC (fro). The antibody is reactive with Rat and Mouse samples.

Quick Overview for SLC6A3 antibody (N-Term) (ABIN215796)

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SLC6A3 Antibody

Binding Specificity: N-Term

Specificity: Recognizes Dopamine transporter (DAT). Species cross-reactivity: Rat and mouse. Weakly reacts with human and canine.

Purification: Immunoaffinity purified

Immunogen: An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (1). This region is presumably the cytoplasmic domain.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC, IHC-Fr, IP, WB (1 - 10 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and ELISA. Western Blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55kD. Immunoprecipitation: 2-10 μg. Immunohistochemistry: 2-10 μg/mL on frozen sections of tissues fixed with 4 % paraformaldehyde. ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide per well.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for SLC6A3

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Alternative Name: SLC6A3 / Dopamine Transporter

Background: Name/Gene ID: SLC6A3
Subfamily: Neurotransmitter:sodium symporter
Family: Transporter

Synonyms: SLC6A3, DA transporter, DAT1, DAT, Dopamine transporter

Gene ID: 6531

UniProt: Q01959

Pathways: Dopaminergic Neurogenesis