SLC6A3 antibody (AA 251-350)

Catalog No. ABIN733373

There are 3+ publications for this product available. The Rabbit Polyclonal anti-SLC6A3 antibody is suitable to detect SLC6A3 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, FACS, IHC (fro), IHC (p), IF (cc) and IF (p).

Quick Overview for SLC6A3 antibody (AA 251-350) (ABIN733373)

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SLC6A3 Antibody

Binding Specificity: AA 251-350

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DAT1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SLC6A3 antibody (ABIN733373)

Romei, Bonifacino, Milanese, Usai, Raiteri: "Colocalization of neurotransmitter transporters on the plasma membrane of the same nerve terminal may reflect cotransmission." in: Brain research bulletin, Vol. 127, pp. 100-110, (2016) (PubMed).

Qian, Cai, Wang, Zhang, Zhao, Qian, Yang, Liu, Hasegawa: "Effect of Gingerol on Cisplatin-Induced Pica Analogous to Emesis Via Modulating Expressions of Dopamine 2 Receptor, Dopamine Transporter and Tyrosine Hydroxylase in the Vomiting Model of Rats." in: Yonago acta medica, Vol. 59, Issue 2, pp. 100-10, (2016) (PubMed).

Ni, Hu, Ren, Luo, Li, Wan, Su: "Self-assembling peptide nanofiber scaffolds enhance dopaminergic differentiation of mouse pluripotent stem cells in 3-dimensional culture." in: PLoS ONE, Vol. 8, Issue 12, pp. e84504, (2013) (PubMed).

Target Details for SLC6A3

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Alternative Name: Dat/Dopamine Transporter

Background:

Synonyms: DAT, DAT1, PKDYS, Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3

Background: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.

Gene ID: 6531

UniProt: Q01959

Pathways: Dopaminergic Neurogenesis