SLC6A3 antibody (pThr53)

Catalog No. ABIN6254067

There are 2+ publications for this product available. The Rabbit Polyclonal anti-SLC6A3 antibody is suitable to detect SLC6A3 in samples from Rat. It has been validated for WB and ELISA.

Quick Overview for SLC6A3 antibody (pThr53) (ABIN6254067)

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SLC6A3 Antibody

Binding Specificity: pThr53

Specificity: Specific for endogenous levels of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Mouse, Rat

Characteristics: Our Dopamine Transporter (Thr53) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Dopamine Transporter (Thr53) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of rat dopamine transporter, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-SLC6A3 antibody (ABIN6254067)

Aher, Subramaniyan, Shanmugasundaram, Sase, Saroja, Holy, Höger, Beryozkina, Sitte, Leban, Lubec: "A Novel Heterocyclic Compound CE-104 Enhances Spatial Working Memory in the Radial Arm Maze in Rats and Modulates the Dopaminergic System." in: Frontiers in behavioral neuroscience, Vol. 10, pp. 20, (2016) (PubMed).

Karabacak, Sase, Aher, Sase, Saroja, Cicvaric, Höger, Berger, Bakulev, Sitte, Leban, Monje, Lubec: "The effect of modafinil on the rat dopamine transporter and dopamine receptors D1-D3 paralleling cognitive enhancement in the radial arm maze." in: Frontiers in behavioral neuroscience, Vol. 9, pp. 215, (2015) (PubMed).

Target Details for SLC6A3

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Alternative Name: SLC6A3

Background:

Synonyms: DA transporter antibody, DAT 1 antibody, DAT antibody, DAT1 antibody, Dopamine transporter 1 antibody, Dopamine transporter antibody, PKDYS antibody, SC6A3_HUMAN antibody, SLC6A3 antibody, Sodium dependent dopamine transporter antibody, Sodium-dependent dopamine transporter antibody, Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 antibody, Solute carrier family 6 (neurotransmitter transporter) member 3 antibody, Solute carrier family 6 member 3 antibody, Variable number tandem repeat (VNTR) antibody

Description Logic: Our Dopamine Transporter (Thr53) rabbit polyclonal phosphospecific primary antibody is produced . It detects Dopamine Transporter (Thr53) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: SLC6A3

Molecular Weight: 55 kDa

Gene ID: 24898

UniProt: P23977

Pathways: Dopaminergic Neurogenesis