Donkey anti-Rabbit IgG Antibody (FITC)

Catalog No. ABIN2176501

Product Details

Target: IgG

Reactivity: Rabbit

Host: Donkey

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Purification: Purified by Protein A.

Isotype: IgG

Application Details

Application Notes: IF(IHC-P): (1:500-2000), IF(IHC-F): (1:500-2000), IF(ICC): (1:500-1000)
Optimal working dilution should be determined by the investigator.

Comment:

Exitation/Emission: 494nm/518nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4 °C for 12 months.

References

Chen, Liu, Lu, Zhu, Tang, Wang, Lu: "Functional roles of C/EBPα and SUMO‑modification in lung development." in: International journal of molecular medicine, Vol. 40, Issue 4, pp. 1037-1046, (2018) (PubMed).

Gao, Liu, Xie, Wang, Qi: "Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 515-522, (2018) (PubMed).

Song, Shah, Yang, Dong, Yang, Zhou, Zhao: "Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models." in: Frontiers in molecular neuroscience, Vol. 10, pp. 128, (2017) (PubMed).

Gao, Jiang, Zhao, Wu, Tian, Ou, Zhang, Pan, Lu, Zhang: "Activation of Autophagy Contributes to the Angiotensin II-Triggered Apoptosis in a Dopaminergic Neuronal Cell Line." in: Molecular neurobiology, Vol. 53, Issue 5, pp. 2911-2919, (2016) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.