RBX1 antibody (C-Term)

Catalog No. ABIN218525

The Rabbit Polyclonal anti-RBX1 antibody is suitable to detect RBX1 in samples from Human. It has been validated for WB, IHC, IP, ELISA and IHC (p).

Quick Overview for RBX1 antibody (C-Term) (ABIN218525)

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RBX1 Antibody

Binding Specificity: C-Term

Specificity: Amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH.

Purification: Delipidated and defibrinated

Immunogen: Synthetic peptide from human RBX1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), IHC, IHC-P (1:500), IP, WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: ROC1 / RBX1

Background: Name/Gene ID: RBX1

Synonyms: RBX1, Regulator of cullins 1, RING box protein 1, ROC1, TESTICAN, Ring-box 1, RNF75, BA554C12.1, Protein ZYP, RING finger protein 75, RING-box protein 1, ZYP protein

Gene ID: 9978

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome