Lipin 1 antibody (AA 111-126)

Catalog No. ABIN6736492

Product Details anti-Lipin 1 Antibody

Target: Lipin 1 (LPIN1)

Binding Specificity: AA 111-126

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lipin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Recognizes mouse Lipin 1. Species sequence Homology: rat (93 % ), human and chimpanzee (81 %), chicken (75 %). Epitope is around 120aa region that is well before the truncation site for isoform 1, therefore epitope is present in both isoforms 1 and 2. No significant sequence homology seen with Lipin-2, Lipin-3 or other proteins.

Purification: Immunoaffinity purified

Immunogen: Synthetic 16aa peptide sequence (aa111-126) at the N-terminus of mouse Lipin 1 (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IF, WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-10 μg/mL using ECL. Will recognize ~140kD mouse Lipin-1 under non-reducing conditions. It is not clear if isoform 2 is also recognized with this antibody. ELISA: 0.5-1 μg/mL. Control peptide can be used to coat ELISA plates at 1 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as liquid in PBS,  pH 7.5, 0.1 % BSA, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Lipin 1

Target: Lipin 1 (LPIN1)

Alternative Name: LPIN1 / Lipin 1 (LPIN1 Products)

Synonyms: LPIN1 antibody, pap1 antibody, PAP1 antibody, 4631420P06 antibody, Kiaa0188 antibody, Lipin1 antibody, fld antibody, mKIAA0188 antibody, zgc:194552 antibody, zgc:194558 antibody, lipin1 antibody, lipin 1 antibody, phosphatidate phosphatase LPIN1 antibody, LPIN1 antibody, lpin1 antibody, LOC100539289 antibody, Lpin1 antibody

Background: Name/Gene ID: LPIN1

Synonyms: LPIN1, Lipin-1, Lipin 1, PAP1, KIAA0188

Gene ID: 23175

UniProt: Q14693

Pathways: Monocarboxylic Acid Catabolic Process