KAT2B antibody (AA 817-832)

Catalog No. ABIN229084

Product Details anti-KAT2B Antibody

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Binding Specificity: AA 817-832

Reactivity: Human, Mouse, Dog, Cow, Horse, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAT2B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes human and canine PCAF. Species cross-reactivity: Predicted to react with mouse and chicken due to sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%) Rat, Opossum, Platypus, Xenopus (94%) Chicken, Lizard, Zebrafish (88%) Stickleback, Pufferfish (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide [EKFFFSKIKEAGLIDK] (with cysteine added to N-terminal) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of human PCAF. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%), Rat, Opossum, Platypus, Xenopus (94%), Chicken, Lizard, Zebrafish (88%), Stickleback, Pufferfish (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (1:500)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500, Determined using whole extracts of human epidermoid carcinoma A431, canine MDCK kidney cells or recombinant active human PCAF, and a chemiluminescent detection reagent. Predicted molecular weight: ~88kD. In some extract preparations, additional band (s) may appear. Staining of PCAF band (s) by immunoblotting is inhibited by the PCAF immunizing peptide. Immunoprecipitation: 5 μg immunoprecipitates PCAF from RIPA lysate with ~4x10^7 human acute T cell leukemia Jurkat cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 15 mM sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KAT2B

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Alternative Name: KAT2B / PCAF (KAT2B Products)

Synonyms: PCAF antibody, pcaf antibody, si:ch211-1j13.2 antibody, zgc:161980 antibody, gb:dq017634 antibody, im:7156024 antibody, CAF antibody, P antibody, P/CAF antibody, A930006P13Rik antibody, AI461839 antibody, AW536563 antibody, Pcaf antibody, p/CAF antibody, lysine acetyltransferase 2B antibody, K(lysine) acetyltransferase 2B antibody, K(lysine) acetyltransferase 2A antibody, KAT2B antibody, kat2b antibody, kat2a antibody, Kat2b antibody

Background: Name/Gene ID: KAT2B

Synonyms: KAT2B, CREBBP-associated factor, GCN5, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, Lysine acetyltransferase 2B, Histone acetylase PCAF, PCAF, p300/CBP-associated factor, CAF, GCN5L

Gene ID: 8850

UniProt: Q92831

Pathways: p53 Signaling, Regulation of Carbohydrate Metabolic Process