CENPA antibody (AA 4-17)
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- Target See all CENPA Antibodies
- CENPA (Centromere Protein A (CENPA))
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Binding Specificity
- AA 4-17
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Reactivity
- Human, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CENPA antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Rabbit (93%) Nectri (86%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. KLH conjugated. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Rabbit (93%), Nectri (86%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG
- Top Product
- Discover our top product CENPA Primary Antibody
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- Application Notes
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Approved: WB
Usage: Suitable for use in Western Blot analysis. Western Blot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20 μg control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2 μg/mL anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 5 mg/mL BSA, 0.05 % sodium azide, 30 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- CENPA (Centromere Protein A (CENPA))
- Alternative Name
- CENPA / CENP-A (CENPA Products)
- Synonyms
- CENP-A antibody, CenH3 antibody, Cenp-A antibody, cenpx antibody, cenp-a antibody, sim2 antibody, Cenp-a antibody, RGD1563607 antibody, CENPA antibody, centromere protein A antibody, centromere protein-A antibody, centromeric histone-3 like protein antibody, centromere-specific histone H3 CENP-A antibody, cenp-A antibody, centromere protein A L homeolog antibody, histone H3-like centromeric protein A antibody, CENPA antibody, Cenpa antibody, CENP-A antibody, cenpa antibody, cenp-A antibody, cnp1 antibody, HAN_3g422 antibody, Cenp-a antibody, cenpa.L antibody, CMU_016480 antibody
- Background
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Name/Gene ID: CENPA
Synonyms: CENPA, Centromere autoantigen A, Centromere-specific histone, CenH3, Centromere protein A, Centromere protein A, 17 kDa, CENP-A, Centromere protein A (17kD) - Gene ID
- 1058
- UniProt
- P49450
- Pathways
- Chromatin Binding, Maintenance of Protein Location
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