Microtubule Associated Protein 2 A/b (MAP2a/b) antibody

Antigen: Microtubule Associated Protein 2 A/b (MAP2a/b)

Clonality: Monoclonal (AP20)

Host: Mouse

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN235804

Additional Information

Alternative name: Microtubule Associated Protein-2 (MAP-2) (2a + 2b)

Format: Purified, Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: AP20

Description: MAb to MAP-2 (2a + 2b) Monoclonal Antibody to Microtubule Associated Protein-2 (MAP-2) (2a + 2b) Cell culture

Specificity: Anti-MAP-2 recognizes (Mr 300kDa) MAP-2 protein from bovine brain. Strongly cross-reacts with MAP-2 from human, rat, mouse and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Reacts with dendrites and cell bodies of neurons.

Application Details

Application Notes: Can be used in protein blotting and immunohistochemistry, frozen tissues only. Anti-MAP-2 may be used in studies of neuron structure in brain tissue. The suggested working dilution for immunohistochemical staining is approximately 1:500. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 100ug/ml (OD280nm)

Purification: Protein G chromatography. Source: Cell culture

Buffer: 0.01M PBS, pH 7.2 containing 1% BSA. Preservative: 0.09% Sodium azide

Storage: Short-term (up to 1 month) store at 2-8°C. Long-term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

Restrictions: For Research Use only