Polyvalent for Shigella antigens. Reacts with Shigella species including sonnei. Antiserum is not absorbed and does react with related Enterobacteriaceae.
Characteristics
Rabbit anti Shigella sp., Rabbit antibody to Shigella species
Purification
IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of Biotin under mild conditions to give a high degree of substitution.
Immunogen
Mixture of S. boydii (ATCC 8700), S. flexneri (ATCC 29903) and S. dysenteriae (ATCC 13313)
Suitable for use in IFA. Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100 % Acetone for 5 minutes at room temperature. Air dry. Stain. It is also suitable for conjugation purposes. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Liquid
Buffer
0.01 M PBS, pH 7.2 Product contains no stabilizing proteins, 0.1 % Sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Short-term (up to 6 months) store at 2-8 °C. Long term, aliquot and store at -28 °C.